可沉微藻轉(zhuǎn)化油脂潛力及PHB合成試驗(yàn)研究
中國給水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 36 期數(shù): 2020年07期 頁碼: 1-6 欄目: 出版日期: 2020-04-01
- Title:
- Lipids Conversion Potential and PHB Synthesis of Settleable Microalgae
- Keywords:
- settleable microalgae; secondary effluent; polymer; lipids; poly-β-hydroxybutyrate (PHB); cyanobacteria
- 摘要:
- 可沉微藻在去除二級(jí)出水中氮、磷的同時(shí)，可以合成并積累多種具有高附加值的聚合物，如油脂、蛋白質(zhì)、多糖、聚β-羥基丁酸酯（PHB）等。前期試驗(yàn)在“沖淘”壓力下篩選出了沉淀率高達(dá)97%的可沉微藻，并通過一系列環(huán)境變化（通入CO2、實(shí)行光/暗循環(huán)等）刺激油脂聚積，最高可獲得49.2%（細(xì)胞干質(zhì)量，下同）的油脂含量。進(jìn)一步強(qiáng)化試驗(yàn)顯示，以增加光照強(qiáng)度與CO2通入量達(dá)到進(jìn)一步提高可沉微藻油脂含量的做法事與愿違，反而出現(xiàn)油脂含量下降的現(xiàn)象。而可沉藍(lán)藻具有相當(dāng)?shù)暮铣伞⒎e累PHB的能力，只要存在少許特異性碳源（乙酸鹽）刺激，可沉藍(lán)藻在不通入CO2的情況下即可輕松合成并積累含量高達(dá)30%左右的PHB。這種主要以無機(jī)碳源合成PHB的方法比以有機(jī)碳源合成PHB的活性污泥法更具吸引力。
- Abstract:
- Beyond removing nitrogen and phosphorous from secondary effluent, the settleable microalgae can also synthesize and accumulate such high value-added polymers as lipids, proteins, polysaccharides, poly-β-hydroxybutyrate (PHB), etc.The previous experiments efficiently enriched settleable microalgae with the settleable rate of more than 97% by applying “washout” pressure, and accumulated the highest lipids content of 49.2% (dry cell weight) by changing environmental factors like CO2, light/dark shift, etc. According to the following experiment results, increasing the light intensity and CO2 intake would not further increase the lipids content of the settleable microalgae. Instead, the lipids content decreased. However, settleable cyanobacteria had a good ability to synthesize and accumulate PHB. As long as there was a tiny specific carbon source (acetate) stimulation, cyanobacteria could easily synthesize and accumulate PHB with the content of up to 30% without CO2 input.Clearly, synthesizing PHB mainly from inorganic carbon source was more attractive than that from organic carbon source by activated sludge.
相
似文獻(xiàn)/References:
[1]鎮(zhèn)祥華,余琴芳,萬年紅,等.污水處理廠二級(jí)出水深床濾池超深度脫氮研究[J].中國給水排水,2020,36(11):91.
[2]雷晨,許路,張瓊?cè)A,等.紫外活化過硫酸鹽技術(shù)用于污水廠二級(jí)出水深度處理[J].中國給水排水,2020,36(15):86.
LEI Chen,XU Lu,ZHANG Qiong-hua,et al.Advanced Treatment of Secondary Effluent from a Wastewater Treatment Plant by Ultraviolet Activated Persulfate[J].China Water & Wastewater,2020,36(07):86.
